Sackler School of
Graduate Biomedical Sciences

 
       
    Liscum Lab Methods  
   

Amphotericin B selection

Cholesterol esterification

Cholesterol transport – Lyso to PM

Cholesterol transport – PM to ER

• Fatty acid synthesis

Filipin staining of cultured cells

• "Gas Chromatography and Lipids" by Wm W. Christie. 1989. The Oily Press, Ltd.

Lipid extraction

Preparation of LDL labeled with [3H]cholesteryl oleate

• Preparation of [3H]oleate

Sterol synthesis

 

 
   

For further info, email Laura