January 2004, Issue 1

The Tufts/Tufts-NEMC Expression Analysis Core (TEAC)

The Tufts/Tufts-NEMC Expression Analysis Core (TEAC) is designed to provide to investigators at Tufts University and Tufts-New England Medical Center the most modern techniques for analyzing gene expression. Equipment and technical support are available both to investigators who have no experience with gene expression analysis and to those who are already familiar with the techniques but could benefit by standardizing and optimizing their experimental protocols and by using standardized supplies.

TEAC was created with and is currently supported by funds from the National Eye Institute (NEI). TEAC also receives institutional support from the Departments of Ophthalmology and Anatomy. TEAC maintains cooperative initiatives with several centers — the Center for Gastroenterology Research on Absorptive and Secretory Processes (GRASP), funded by the National Institute of Diabetes and Digestive and Kidney Disorders (NIDDK); the Center for Neuroscience Research (CNR), funded by the National Institute of Neurological Disorders and Stroke (NINDS), and the Tufts Center for Vision Research (TCVR), funded by NEI. Together, these sources have created a facility that is currently available to the entire Tufts University and Tufts-New England Medical Center research community for use on a fee-for-service basis.

TEAC is located on the Boston health sciences campus in the M&V (medical and veterinary) complex, within the Medical School's Department of Anatomy and Cellular Biology. Available equipment includes an MJ Research Tetrad PCR cycler, a BioRobotics MicroGrid II arrayer, a Packard Biochip 4000 2-color confocal scanner, robotic liquid handlers for additional automation, and a full range of ancillary laboratory and computer equipment including image (Quantarray, ImaGene) and data (GeneSpring) analysis software. A Stratagene MX4000 multiplexing real-time PCR cycler is available for accurate simultaneous assessment of several RNA levels from many samples.

TEAC offers standard arrays produced in-house for mouse, human, and drosophila genes, as well as custom arrays. TEAC also offers discounted pricing on Affymetrix GeneChips. Standardized labeling, hybridization, and scanning services are provided by TEAC staff supervised by Lan Wei. Through a collaboration between the CNR and the TCVR, bioinformatics and other computer support are provided by Emily Lyettefi, under the direction of Kurt Wollenburg, PhD, assistant professor of ophthalmology.

For more information about TEAC, go to http://www.tufts.edu/med/teac/.

For information on other core facilities at Tufts, go to http://www.tufts.edu/central/research/CoreFacilities.htm



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