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Amy S. Yee

Jaharis 614
150 Harrison Ave.
Boston, MA 02111
amy.yee@tufts.edu

office: 617-636-6850
lab: 617-636-6851

Amy S. Yee, Ph.D.

Professor,Tufts Department of Biochemistry

 

Links:

Research

Lab Members

Recent Publications

  

Research Summary:

Our lab is investigating the functions of the HBP1 gene in cell cycle inhibition and in tumor suppression. HBP1 is a transcriptional repressor and cell cycle regulator. We previously cloned HBP1 as an interactor with the retinoblastoma family, but now have shown a diverse role in regulating proliferation and cancer. Some of the gene targets include Cyclin D1 and N-MYC. Recent work has shown that HBP1 is a suppressor of Wnt signaling pathway, which has emerged as extensively mutated in cancer. Dysregulation of Wnt signaling is correlated with breast, liver, colon, and other cancers. Consistent with tumor suppression, the HBP1 gene resides in chromosome 7q31, which is a region mutated in cancer. We are using genetic and functional approaches to delineate the basis of HBP1 mutations in breast and other cancers. We are investigating the feasibility of HBP1 as a novel diagnostic and therapeutic target in cancer. Our long-term goal is the use of fundamental information to make a concrete contribution to new molecular tools for cancer treatment and diagnosis.

Research:

For more details, please see the publications below.

Lab Members

Recent Publications

  1. Rieger-Christ, KM; Ng, L; Hanley, RS; Durrani, O; Ma, H; Yee, AS; Libertino, JA; Summerhayes, IC. (2005). “Restoration of plakoglobin expression in bladder carcinoma cell lines suppresses cell migration and tumorigenic potential.” British Journal of Cancer 92(12): 2153-2159
  2. Kim, Jiyoung; Zhang, Xiaowei; Rieger-Christ, Kimberly M; Summerhayes, Ian C; Wazer, David E; Paulson, K. Eric; Yee, Amy S. (2006). “Suppression of Wnt Signaling by the Green Tea Compound (–)-Epigallocatechin 3-Gallate (EGCG) in Invasive Breast Cancer Cells.” Journal of Biological Chemistry 281(16): 10865-10875
  3. Yee, Amy S; Paulson, Eric K.; McDevitt, Michael A; Rieger-Christ, Kimberly; Summerhayes, Ian; Berasi, Stephen P; Kim, Jiyoung, Huang, Chun-Yin; Zhang, Xiaowei. (2004). "The HBP1 transcriptional repressor and the p38 MAP kinase: unlikely partners in G1 regulation and tumor suppression." Gene 336(1): 1-13.
  4. Yee, A.S., Paulson, K.E., McDevitt, M.A., Rieger-Christ, Summerhayes, I. K. Berasi, S.P., Kim,J. Huang, C.Y., Zhang, X. (2004) Unlikely Partners: the HBP1 Transcriptional Repressor and the p38 Map Kinase in G1 Regulation and Tumor Suppression, GENE. In press.
  5. Berasi, S.P. Xiu, M., Yee, A.S. Paulson, K.E (2004). Mol.Cell. Biol. 24:3011-3024. “HBP1 Repression of the p47 phox gene: Cell cycle regulation via the NADPH Oxidase.”
  6. Sivak, Jeremy M. J. A. West-Mays, A.S.Yee, T. Williams3 and M. E. Fini (2004).Mol. cell. Biol 24:245-257.“Pax6 Coordinates Corneal Re-epithelialization by Controlling Gelatinase B (MMP-9) Expression Through Cooperative Interactions With AP-2.”
  7. Xiu, M., Sampson, E., Huang, C.Y., Davis, R.J., Paulson, K.E., and Yee, A.S. (2003) Mol. Cell. Biol. 23:8890- 8901. The Transcriptional Repressor HBP1 is a Target of the p38 MAP Kinase Pathway”
  8. Yee, A.S and Wang, JY (2003) Role of RB and E2F in G1 progression. (G1 control. Ed. J. Boonstra,; Landes Biosciences) http://www.eurekah.com/chapter.php?chapid=1053&bookid=74&catid=14
  9. Hassan, W.N., Cantuti-Castelevetri, I., Denisova, N., Yee, A.S., Joseph, J.A. and Paulson, K.E. (2002) Free Rad. Biol. Med, 32:551-561"The Nitrone Spin-Trap PBN Alters the Cellular Response to H2O2: Calcium Dependent Activation of the EGF Receptor/ERK Pathways”
  10. Sampson, E., Haque, Z., Ku, Man-Ching, Tevosian, S., Pestell, R.G. and Yee, A.S. (2001) EMBOJ.20: 4500-11 "Negative Regulation of the Wnt-bcatenin Pathway by the Transcriptional Repressor HBP1”
  11. Shih, H. H., Xiu, M., Berasi, S. P., Sampson, E. M., Leiter, A., Paulson, K. E., and Yee, A. S. (2001). HMG box transcriptional repressor HBP1 maintains a proliferation barrier in differentiated liver tissue. Mol Cell Biol 21, 5723-5732. 
  12. Sampson, E., Haque, Z., Ku, Man-Ching, Tevosian, S., Pestell, R.G. and Yee, A.S (2001). Negative regulation of the Wnt-beta-catenin pathway by the transcriptional repressor HBP1. Embo J 20, 4500-4511. 
  13. Yang, R., Muller, C., Huynh, V., Fung, Y. K., Yee, A. S., and Koeffler, H. P. (1999). Functions of cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins. Mol Cell Biol 19, 2400-2407. 
  14. Gartel, A. L., Goufman, E., Tevosian, S. G., Shih, H., Yee, A. S., and Tyner, A. L. (1998). Activation and repression of p21(WAF1/CIP1) transcription by RB binding proteins. Oncogene 17, 3463-3469. 
  15. Yee, A.S., Tevosian, S.G., Shih, H. (1998). " New Perspectives On Retinoblastoma Family Functions In Differentiation." Frontiers in BioSciences 3, d532-547. 
  16. Yee, A.S., Tevosian, S.G., Shih, H. (1999). "Perspectives on Retinoblastoma Family Functions in Differentiation." Pediatric Pathology and Molecular Medicine 18, 275-302. 
  17. Shih, H. H., Tevosian, S. G., and Yee, A. S. (1998). Regulation of differentiation by HBP1, a target of the retinoblastoma protein. Mol Cell Biol 18, 4732-4743. 
  18. Shin, E. K., Tevosian, S. G., and Yee, A. S. (1996). The N-terminal region of E2F-1 is required for transcriptional activation of a new class of target promoter. J Biol Chem 271, 12261-12268. 
  19. Tevosian, S. G., Paulson, K. E., Bronson, R., and Yee, A. S. (1996). Expression of the E2F-1/DP-1 transcription factor in murine development. Cell Growth Differ 7, 43-52. 
  20. Tevosian, S. G., Shih, H. H., Mendelson, K. G., Sheppard, K. A., Paulson, K. E., and Yee, A. S. (1997). HBP1: a HMG box transcriptional repressor that is targeted by the retinoblastoma family. Genes Dev 11, 383-396. 

             

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