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Amy S. Yee

Jaharis 614
150 Harrison Ave.
Boston, MA 02111
amy.yee@tufts.edu

office: 617-636-6850
lab: 617-636-6851

Amy S. Yee, Ph.D.

Professor,Tufts Department of Biochemistry

 

Links:

Research

Lab Members

Recent Publications

  

Research Summary:

Our lab is investigating the functions of the HBP1 gene in cell cycle inhibition and in tumor suppression. HBP1 is a transcriptional repressor and cell cycle regulator. We previously cloned HBP1 as an interactor with the retinoblastoma family, but now have shown a diverse role in regulating proliferation and cancer. Some of the gene targets include Cyclin D1 and N-MYC. Recent work has shown that HBP1 is a suppressor of Wnt signaling pathway, which has emerged as extensively mutated in cancer. Dysregulation of Wnt signaling is correlated with breast, liver, colon, and other cancers. Consistent with tumor suppression, the HBP1 gene resides in chromosome 7q31, which is a region mutated in cancer. We are using genetic and functional approaches to delineate the basis of HBP1 mutations in breast and other cancers. We are investigating the feasibility of HBP1 as a novel diagnostic and therapeutic target in cancer. Our long-term goal is the use of fundamental information to make a concrete contribution to new molecular tools for cancer treatment and diagnosis.

Research:

For more details, please see the publications below.

Lab Members

Recent Publications

  1. Tevosian, S.G., Shih, H., Mendelson, K.G., Sheppard, K.A., Paulson, K.E, and Yee, A.S. (1997) Genes and Dev. 11:383 – 396 "HBP1: a HMG box Transcriptional Repressor that is Targeted by the Retinoblastoma Family"
  2. Shih, H., Tevosian, S., and Yee, A.S. (1998). Mol. Cell. Biol. 18(8):4732-43. "Regulation of Differentiation by HBP1, a Target of the Retinoblastoma Protein"
  3. Yee, A.S., Tevosian, S.G., Shih, H. (1998). Frontiers in BioSciences 3:d532-547. " New Perspectives On Retinoblastoma Family Functions In Differentiation."
  4. Gartel, A.L., Goufman, E., Shih, H., Tevosian, S., Yee, A.S., and Tyner, A.L. (1998). Oncogene, 17:3463-9. "Activation and Repression of p21WAF1/CIP1 transcription by RB binding proteins.”
  5. Yee, A.S., Tevosian, S.G., Shih, H. (1999). Pediatric Pathology and Molecular Medicine 18:275-302. "Perspectives On Retinoblastoma Family Functions In Differentiation."
  6. Yang, Rong, Muller, C., Huynh, V., Fung, Y.K., Yee, A.S., Koeffler, H.K. (1999). Mol. Cell Biol. 19:2400-2407. “Functions of the Cyclin A1 in the Cell Cycle and its Interaction with Transcription Factor E2F1 and the Rb Family of Proteins.”
  7. Shih, H., Xiu, M. Berasi, S., Sampson, E.M. Leiter, A.B., Paulson, K.E, and Yee, A.S. (2001) Mol. Cell Biol. 21:5723-5735. "The HMG Box Transcriptional Repressor HBP Maintains a Proliferation Barrier in Differentiated Liver Tissue"
  8. Sampson, E., Haque, Z., Ku, Man-Ching, Tevosian, S., Pestell, R.G. and Yee, A.S. (2001) EMBOJ.20: 4500-11 "Negative Regulation of the Wnt-bcatenin Pathway by the Transcriptional Repressor HBP1
  9. Hassan, W.N., Cantuti-Castelevetri, I., Denisova, N., Yee, A.S., > Joseph, J.A. and Paulson, K.E. (2002) Free Rad. Biol. Med, 32:551-561 "The Nitrone Spin-Trap PBN Alters the Cellular Response to H2O2: Calcium Dependent Activation of the EGF Receptor/ERK Pathways”
  10. Yee, A.S and Wang, JY (2003) Role of RB and E2F in G1 progression. (G1 control. Ed. J. Boonstra,; Landes Biosciences) http://www.eurekah.com/chapter.php?chapid=1053&bookid=74&catid=14
  11. Xiu, M., Sampson, E., Huang, C.Y., Davis, R.J., Paulson, K.E., and Yee, A.S. (2003) Mol. Cell. Biol. 23:8890-8901. The Transcriptional Repressor HBP1 is a Target of the p38 MAP Kinase Pathway
  12. Sivak, Jeremy M. J. A. West-Mays, A.S.Yee, T. Williams, and M. E. Fini (2004).Mol. cell. Biol 24:245-257. “Pax6 Coordinates Corneal Re-epithelialization by Controlling Gelatinase B (MMP-9) Expression Through Cooperative Interactions With AP-2.”
  13. Berasi, S.P. Xiu, M., Yee, A.S. Paulson, K.E (2004). Mol.Cell. Biol. 24:3011-3024 “HBP1 Repression of the p47 phox gene: Cell cycle regulation via the NADPH Oxidase.”
  14. Yee, A.S., Paulson, K.E., McDevitt, M.A., Rieger-Christ, Summerhayes, I. K. Berasi, S.P., Kim,J. Huang, C.Y., Zhang, X. (2004) Unlikely Partners: the HBP1 Transcriptional Repressor and the p38 Map Kinase in G1 Regulation and Tumor Suppression, Gene 336:1-13.
  15. Rieger-Christ, K.R., Ng, K., Hanley, R.S., Durrani, O, Ma, H., Yee, A.S. Libertino, J.A. and Summerhayes, I.C. (2005). “Restoration of plakoglobin expression in bladder carcinoma cell lines suppresses cell migration and tumorigenic potential”. British Journal of Cancer, 92(12):2153-9
  16. D.E. Wazer, K. Reiger-Christ, M.A. McDevitt, Z. Huang, D. Giri, S. Kaufmann, J. Kim. S.P. Berasi,I. Summerhayes, K.E. Paulson, A.S. Yee (2005) International Journal Radiation Oncology, Biology, Physics Volume 63, Number 2, S252.“Breast-cancer Variants of the HBP1 Transcriptional Repressor: Association with Invasive Breast Cancer.”
  17. J.D. Purviance, J. Kim,2 B.P. Stephan, A.S. Yee, K.E. Paulson, D.E. (2005) Proceedings of the 47th Annual ASTRO Meeting S485. “Loss of the HBP1 Transcriptional Repressor Confers Radiosensitivity to Breast Tumor Cells.”
  18. J. Purviance, J. Kim, B. Stephan, A.S. Yee, K. Paulson, D. Wazer (2005) International Journal of Radiation Oncology Biology Physics, Volume 63, Pages S485-S486. “Loss of the HBP1 Transcriptional Repressor Confers Radiosensitivity to Breast Tumor Cells.”
  19. Kim, J., Zhang. X, Rieger-Christ, K.M., Summerhayes, I.C., Wazer, D.E.,Paulson, K.E., and Yee, A.S. (2006) “Suppression Of Wnt Signaling By The Green Tea Compound EGCG And the HBP1 Transcriptional Repressor In Breast Cancer Cells”. J Biol. Chem. 281:10865
  20. Zhang, X.W., Kim, J., Ruthahzer, R., Wazer, D.E., Paulson, K.E., Yee, A.S. (2006) Mol. Cell Biol. (22):8252-66. “The HBP1 transcriptional repressor participates in RAS-induced premature senescence.”
  21. Paulson, K.E., Rieger-Christ, K.M., McDevitt, M.A., Kuperwasser, C., Kim, J., Zhang, X., Hu, M., Ruthazer, R., Berasi, S.P., Huang, C.Y., Paganelli, R.H., Giri, D., Kaufman, S., Dugan, J., Blum, J., Netto, G., Huang, Z. Wazer, D.E., Summerhayes, I.C., Yee, A.S. (2007) Cancer Research, 67:6136-6145. “Alterations Of The HBP1 Transcriptional Repressor Are Associated With Invasive Breast Cancer.”
  22. Rieger-Christ K.M.1, Hanley, R., Lodowsky, C., Bernier, T., Vemulapalli, P., Roth, M., Kim, J., Yee, A.S., Meé, S. Le, Marie, P.J., Libertino, J.A., and Summerhayes, I.C. (2007) Journal of Cellular Biochemistry, 102-377-388. “The Green Tea Compound, (-)-Epigallocatechin-3-gallate Down-Regulates N-Cadherin and Suppresses Migration of Bladder Carcinoma Cells.”

             

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