Lecture 4  

Gary Sahagian - Import Into Mitochondria & Peroxisomes

Background reading for Lectures 4:

Alberts, Chapter 12 (all sections not already assigned).

Mito Import Review.pdf

Peroxisomal Import Review.pdf

Articles to read after Lecture 4:

Pfanner, N. and Geissler, A. (2001) Versatility of the Mitochondrial Protein Import Machinery, Nature Rev. Mol. Cell Biol. 2, 339-349. (review)

Smith, M.D. and Schnell, D.J. (2001) Peroxisomal Protein Import: The Paradigm Shifts, Cell 105, 293-296. (review)

Other Lecture 4 articles (for reference only):

Reviews - Mitochondria:

  1. Deshaies, R.J., Koch, B.D. and Schekman, R. (1988) “The Role of Stress Proteins in Membrane Biogenesis, TIBS 13, 384-388.

  2. Hartl, F.-U. and Neupert, W. (1990) Protein Sorting to Mitochondria: Evolutionary Conservation of Folding and Assembly, Science 247, 930-938.

  3.  Wickner, W.T. (1994) How ATP Drives Proteins Across Membranes, Science 266, 1197-1198.

  4. Craig, E.A., Weissman, J.S. and Horwich, A.L. (1994) Heat Shock Proteins and Molecular Chaparones: Mediators of Protein Conformation and Turnover in the Cell, Cell 78, 365-372.

  5. Glick, B.S. (1995) Can Hsp70 Proteins Act as Force-Generating Motors? Cell 80, 11-14.

  6. Neupert, W. (1997) Protein Import into Mitochondria, Annu. Rev. Biochem. 66, 863-917.

  7. Matouschek, A., Pfanner, N. and Voos, W. (2000) Protein Unfolding by Mitochondria, EMBO Reports 1, 404-410.

  8. Pfanner, N. and Geissler, A. (2001) Versatility of the Mitochondrial Protein Import Machinery, Nature Rev. Mol. Cell Biol. 2, 339-349.

Original Research Articles - Mitochondria:

  1. Gasser, S.M., Daum, G. and Schatz, G. (1982) Import of Proteins into Mito-chondria: Energy-dependent Uptake of Precursors by Isolated Mitochondria, J. Biol. Chem. 257, 13034-1304 (and accompanying papers).

  2. Pfanner, N. and Neupert, W. (1986) Transport of F1-ATPase Subunits Beta into Mitochondria Depends on Both a Membrane Potential and Nucleoside Triphos-phates, FEBS Let. 209, 152-156.

  3.  Eilers, M., Oppliger, W., and Schatz, G. (1987) Both ATP and an Energized Inner Membrane Are Required to Import a Purified Precursor Protein into Mitochondria, EMBO J. 6, 1073-1077.

  4. Schwaiger, M., Herzog, V. and Neupert, W. (1987) Characterization of Translocation Contact Sites Involved in the Import of Mitochondrial Proteins, J. Cell Biol. 105, 235-246.

  5. Roise, D., Theiler, F., Horvath, S.J., Tomich, J.M., Richards, J.H., Allison, D.S. and Schatz, G. (1988) Amphiphilicity is Essential for Mitochondrial Presequence Function, EMBO J. 7, 649-653.

  6. Pfaller, R., Steger, H.F., Rassow, J., Pfanner, N. and Neupert, W. (1988) Import Pathways of Precursor Proteins into Mitochondria: Multiple Receptor Sites Are Followed by a Common Membrane Insertion Site, J. Cell Biol. 107, 2483-2490.

  7. Pfanner, N., Tropschug, M. and Neupert, W. (1987) Mitochondrial Protein Import: Nucleoside Triphosphates Are Involved in Conferring Import-Competence to Precursors, Cell 49, 815-823.

  8. Deshaies, R.J., Koch, B.D., Werner-Washburne, M., Craig, E.A. and Schekman, R. (1988) A Subfamily of Stress Proteins Facilitates Translocation of Secretory and Mitochondrial Percursor Polypeptides, Nature 332, 800-805.

  9. Cheng, M.Y., Hartl, F.-U., Martin, J., Pollack, R.A., Kalousek, F., Neupert, W., Hallberg, E.M., Hallberg, R.L. and Horwich, A.L. (1989) Mitochondrial Heat-Shock Protein Hsp60 Is Essential for Assembly of Proteins Imported into Yeast Mitochondria, Nature 337, 620-625.

  10. Craig, E.A., Kramer, J., Shilling, J. and Nicolet, C.M. (1989) SSC1, an Essential Member of the Yeast HSP70 Multigene Family, Encodes a Mitochondrial Protein, Mol. Cell. Biol. 9, 3000-3008.

  11.  Vestweber, D., Brunner, J., Baker, A. and Schatz, G. (1989) A 42K Outer-Membrane Protein is a Component of the Yeast Mitochondrial Protein Import Site, Nature 341, 205-209.

  12.  Sollner, T., Griffiths, G., Pfaller, R., Pfanner, N.,and Neupert, W. (1989) MOM19, an Import Receptor for Mitochondrial Precursor Proteins, Cell 59, 1061-1070.

  13.  Kiebler, M., Pfaller, R., Sollner, T., Griffiths, G., Horstmann, H., Pfanner, N. and Neupert, W. (1990) Identification of a Mitochondrial Receptor Complex Required For Recognition and Membrane Insertion of Precursor Proteins, Nature 348, 610-616.

  14.  Kang, P.J., Ostermann, J., Shilling, J., Neupert, W., Craig, E.A. and Pfanner, N. (1990) Requirement for hsp70 in the Mitochondrial Matrix for Translocation and Folding of Precursor Proteins, Nature 348, 137-143.

  15. Manning-Krieg, U.C., Scherer, P.E. and Schatz, G. (1991) Sequential Action of Mitochondrial Chaparones in Protein Import into the Matrix, EMBO J. 10, 3273-3280.

  16. Glick, B.S., Brandt, A., Cunningham, K., Muller, S., Hallberg, R.L. and Schatz, G. (1992) Cytochrome c1 and b2 are Sorted to the Intermembrane Space of Yeast Mitochondria by a Stop-transfer Mechanism, Cell 69, 809-822.

  17. Horst, M., Hilfiker-Rothenfluh, S., Opplinger, W. and Schatz, G. (1995) Dynamic Interaction of the Protein Translocation Systems in the Inner and Outer Membranes of Yeast Mitochondria, EMBO J. 14, 2293-2297.

  18. Rojo, E.E., Stuart, R. and Neupert, W. (1995) Conservative Sorting of Fo-ATPase Subunit 9: Export from Matrix Requires Delta pH Across Inner Membrane and Matrix ATP, EMBO J. 14, 3445-3451.

Reviews - Peroxisomes:

  1. Subramani, S. (1993) Import of Proteins into Peroxisomes and Biogenesis of the Organelle, Annu. Rev. Cell Biol. 9, 447-478.

  2. Subramani, S. (1996) Convergence of Model Systems for Peroxisome Biogenesis, Current Opin. Cell Biol. 8, 513-518.

  3.  Terlecky, S.R., Nuttley, W.M. and Subramani, S. (1996) The Cytosolic and Membrane Components Required for Peroxisomal Protein Import, Experientia 52, 1050-1054.

  4. Smith, M.D. and Schnell, D.J. (2001) Peroxisomal Protein Import: The Paradigm Shifts, Cell 105, 293-296.

Original Research Articles - Peroxisomes:

  1. Gould, S., Keller, G.-A. and Subramani, S. (1987) Identification of a Peroxisomal Targeting Signal at the Carboxy Terminus of Firefly Luciferase, J. Cell. Biol. 105, 2923-2932.

  2. Gould, S., Keller, G.-A., Hosken, N., Wilkinson, J. and Subramani, S. (1987) A Conserved Tripeptide Sorts Proteins to Peroxisomes, J. Cell. Biol. 105, 2923-2932.

  3. Brul, S., Westerveld, A., Strijland, A., Wanders, R.J.A., Schram, A.W., Heymans, H.S.A., Schutgens, R.B.H., van den Bosch, H. and Tager, J.M. (1992) Genetic Heterogeneity in the Cerebrohepatorenal (Zellweger) Syndrome and Other Inherited Disorders with a Generalized Impairment of Peroxisomal Functions, J. Clin. Invest. 81, 1710-1715.

  4. McCollum, D., Monosov, E. and Subramani, S. (1993) The pas8 Mutant of Pichia pastoris Exhibits the Peroxisomal Protein Import Deficiencies of Zellwager Syndrome Cells ­The PAS8 Protein Binds to the COOH-Terminal Tripeptide Peroxisomal Targeting Signal, and Is a Member of the TPR Protein Family, J. Cell, Biol. 121, 761-774.

  5. McNew, J.A. and Goodman, J.M. (1994) An Oligomeric Protein Is Imported into Peroxisomes In Vivo, J. Cell Biol. 127, 1245-1257.

  6. Walton, P.A., Hill, P.E. and Subramani, S. (1995) Import of Stably Folded Proteins into Peroxisomes, Mol. Biol. Cell 6, 675-683.

  7. Terlecky, S.R., Nuttley, W.M., McCollum, D., Sock, E. and Subramani, S. (1995) The Pichia pastoris Peroxisomal Protein PAS8p Is the Receptor for the C-terminal Tripeptide Peroxisomal Targeting Signal, EMBO J. 14, 3627-3634.

  8. Fransen, M., Brees, C., Baumgart, E., Vanhooren, J.C., Baes, M., Mannaerts, G.P. and Van Veldhoven, P. (1995) Identification and Characterization of the Putative Human Peroxisomal C-Terminal Targeting Signal Import Receptor, J. Biol. Chem. 270, 7731-7736.