Principal Investigator:
John Castellot, Ph.D.
Professor and Director of the CMDB Graduate Program
Department of Anatomy and Cellular Biology

Department of Anatomy & Cellular Biology
Tufts University 
School of Medicine
136 Harrison Avenue
Boston, Massachusetts 02111

Office Phone:  
(617)636-0303
Lab Phone: 
(617)636-
FAX: 
(617)636-6536

EMail Address:
john.castellot@tufts.edu


Program in Cellular and Molecular Biology

Sackler School

Medical School

Fig. 10 CCN5 is secreted and sticks tightly to the cell surface. Using an antibody that specifically recognizes CCN5, we looked at localization of CCN5 in both permeabilized (membrane extracted) and non-permeabilized SMC. CCN5 is red, the actin cytoskeleton is green, and the nucleus is blue. The pattern seen in membrane-extracted cells is highly indicative of a secreted protein, with its strong perinuclear (Golgi) staining and individual small vesicles seen in the cytoplasm. In non-permeabilized cells, CCN5 is not distributed throughout the matrix and instead appears to adhere tightly to the cell surface. This pattern is termed “matricellular”.