Principal Investigator:
John Castellot, Ph.D.
Professor and Director of the CMDB Graduate Program
Department of Anatomy and Cellular Biology

Department of Anatomy & Cellular Biology
Tufts University 
School of Medicine
136 Harrison Avenue
Boston, Massachusetts 02111

Office Phone:  
(617)636-0303
Lab Phone: 
(617)636-
FAX: 
(617)636-6536

EMail Address:
john.castellot@tufts.edu


Program in Cellular and Molecular Biology

Sackler School

Medical School

Fig. 12 CCN5 knockdown increases motility rates in SMC. When siRNA is used to reduce endogenous CCN5 levels, the motility of SMC in a scratch-wound assay is increased significantly compared to untreated cells or cells transfected with an irrelevant (control) siRNA.

Fig. 13 CCN5 regulates the actin cytoskeleton. Normally, SMC have a very prominent actin filament system, as seen in untreated cells. CCN5 knockdown causes more than half the cells to display greatly reduced actin staining. Quantitative PCR analysis indicates that CCN5 knockdown causes a 50% reduction in actin mRNA levels, consistent with the immunolocalization studies