Principal Investigator:
John Castellot, Ph.D.
Professor and Director of the CMDB Graduate Program
Department of Anatomy and Cellular Biology

Department of Anatomy & Cellular Biology
Tufts University 
School of Medicine
136 Harrison Avenue
Boston, Massachusetts 02111

Office Phone:  
(617)636-0303
Lab Phone: 
(617)636-
FAX: 
(617)636-6536

EMail Address:
john.castellot@tufts.edu


Program in Cellular and Molecular Biology

Sackler School

Medical School

Fig. 15 Two animal models to study estrogen-CCN5 interactions. Normal rats have a 4-day menstrual cycle, which means that it cycles from high estrogen in proestrus to low estrogen in metestrus every 2 days. We can also ovariectomize the rat (remove the ovaries), which drops estrogen and progesterone levels to near zero within 10 days. At that time, it is possible to add back estrogen (in the form of estradiol benzoate), progesterone, or both and measure uterine CCN5 levels in response.