Principal Investigator:
John Castellot, Ph.D.
Professor and Director of the CMDB Graduate Program
Department of Anatomy and Cellular Biology

Department of Anatomy & Cellular Biology
Tufts University 
School of Medicine
136 Harrison Avenue
Boston, Massachusetts 02111

Office Phone:  
(617)636-0303
Lab Phone: 
(617)636-
FAX: 
(617)636-6536

EMail Address:
john.castellot@tufts.edu


Program in Cellular and Molecular Biology

Sackler School

Medical School

Fig. 16 Proestrus rats express more CCN5 than metestrus rats. Immunohistochemistry using the specific anti-CCN5 antibody demonstrates high levels of CCN5 throughout the uterus of proestrus rats compared to metestrus animals. This observation is corroborated by Western blot analysis and by quantitative PCR analysis.

Fig. 17 Estrogen but not progesterone stimulates CCN5 in ovariectomized (OVX) rats. Ovariectomized rats were given estrogen in the form of estradiol benzoate (EB), progesterone (P), or both, dissolved in a mineral oil base. Estrogen strongly induced CCN5 expression throughout the uterus, whereas progesterone neither induced CCN5 nor inhibited its induction by estrogen.