Principal Investigator:
John Castellot, Ph.D.
Professor and Director of the CMDB Graduate Program
Department of Anatomy and Cellular Biology

Department of Anatomy & Cellular Biology
Tufts University 
School of Medicine
136 Harrison Avenue
Boston, Massachusetts 02111

Office Phone:  
(617)636-0303
Lab Phone: 
(617)636-
FAX: 
(617)636-6536

EMail Address:
john.castellot@tufts.edu


Program in Cellular and Molecular Biology

Sackler School

Medical School

Fig. 6 CCN5 expression in the healthy and injured artery wall. Using a highly specific antibody that recognizes CCN5, we see high levels of CCN5 in the medial layers of the healthy rat aorta, in which the SMC are non-proliferating. As shown in the control panels, the IEL and other elastic laminae autofluoresce bright red, making it easy to see the CCN5 staining in between the laminae. Two days after the angioplasty, the SMC are actively traversing the cell cycle, though they have yet to undergo significant cell division. Note the nearly complete absence of CCN5 in these actively proliferating SMC. By 14 days after the injury, the lesion (outlined in white) is complete and the SMC are again non-proliferating. CCN5 is now strongly evident in both the vessel wall and in the lesion.