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| Rob Willson, PhD
Dept. Anatomy and Cellular Biology
M&V 504
136 Harrison Avenue
Boston, MA 02111 |
Tel:
(617) 636-6607
Fax: (617) 636-6536
rwillson@emerald.tufts.edu |
| The Tufts
confocal Microscope Facility is located within the Department
of Anatomy and Cellular Biology and is in its fourth year
of operation. Services are provided for receiving living
or fixed samples labeled with 1-3 distinct fluorochromes
with the most popular application being 3D reconstructions
from optical sections, 2 or 3 overlay analysis for analyzing
spatial proximity and improved spatial resolution with
thick specimens. The fee-for-service facility provides
training, various image processing/analysis opportunities,
and hard copy output for publication or presentation purposes. |
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| Cathy Linsenmayer
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Tel:
(617) 636-0842
cathy.linsenmayer@tufts.edu
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Elizabeth Benecchi
Dept. Anatomy and Cellular Biology
136 Harrison Avenue
Boston, MA 02111 |
Tel: (617)
636-6929
lbenecch@opal.tufts.edu |
| The Electron
Microscope Applications Facility has become a university
resource. as evidenced by its utilization by the Departments
of Physiology, Microbiology, Neuroscience, NEMC Opthalmology,
Surgical Research, Nephrology, Gastroenterology, Chemistry
(Medford), the HNRC and MGH Surgery. It includes the Electron
Microscopy Laboratory (M&V 142) and a Microscopy
Suite (M&V 504) and as of 1999, has been directed
by the very capable and talented duo of Cathy Linsenmayer
and Liz Benecchi.
The Electron Microscope Laboratory
us equipped to prepare materials for conventional transmission
and scanning electron microscopy, and to provide such
specialized techniques as immunoelectron microscopy using
pre-embedding techniques and post-embedding approaches
using ultra thin cryo sections which current users and
staff routinely employ. The facility also supports molecular
structure analysis and epitope mapping of antibody binding
using electron microscopy of rotary shadowed and negatively
stained preparations, and a variety of photographic procedures
including printing of micrographs, production of plates
for publication and slide preparation.
This fee-for-service is open to researchers
in the Tufts Health Sciences Campus who wish to visualize
their samples at the sub-cellular level.The facility offers
support for fixation, embedding, sectioning, staining and
microscope use |
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| Rob
Willson, PhD
Dept. ANatomy and Cellular Biology
M&V 504
136 Harrison Avenue
Boston, MA 02111 |
Tel:
(617) 636-6607
Fax: (617) 636-6536
rwillson@emerald.tufts.edu |
| The
Tufts Microscopy & Digital Image Analysis Facility
is located within the Department of Anatomy and Cellular
Biology. Services are provided for imaging bright-field,
dark-field, phase-contrast, or fluorescently labeled samples.
Current equipment inclues a Zeiss Axioscope and a high-resolution
(1600 x 1200 pixels) Diagnostic Imaging Color Digital camera;
a CCD video camera and Image Intensifier is also available
for real-time biological imaging of live specimens. Quantitation
of images may be done using OPTIMAS image analysis software
which runs on a Pentium-III workstation. |
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| Andrew Plaut, M.D.,
Director
Division of Gastroenterology
Department of Medicine
New England Medical Center
750 Washington Street
Boston, MA 02111
136 Harrison Avenue
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Tel:
(617) 636-6249
Fax: (617) 636-7783
monica.russell@es.nemc.org |
| The Tufts/NEMC
Center for Gastroenterology Research on Absorptive and
Secretory Processes (GRASP) was established in 1984
to promote research in digestive diseases on the Tufts Health
Sciences Campus. Collaborating GRASP scientists are members
of Departments of Tufts University Schools of Medicine and
Veterinary Medicine, New England Medical Center Hospitals
(NEMC), and the USDA Human Nutrition Research Center. GRASP
has become the prototype of successful multi-disciplinary
research interactions among investigators at the hospital
and the Tufts health sciences schools. Despite the independence
of these institutions, the Center has brought together basic
and clinical scientists in a tightly coordinated and productive
program. The GRASP Center consists of five
Core Laboratories that service approximately 40 NIH
funded investigators, whose studies represent the research
base of the Center. |
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| Michael Berne, PhD
Dept. Physiology
Stearns 808
136 Harrison Avenue
Boston, MA 02111 |
Tel:
(617) 636-2422
Fax: (617) 636-6737
michael.berne@tufts.edu |
| The Tufts DNA
Sequencing, DNA Synthesis, and Peptide Synthesis Facility
is located within the Department of Physiology. The
DNA sequencing lab consists of three automated ABI 37X DNA
sequencers. Each instrument runs 36 samples with a read
length of 500-800 bases. The turnaround time is generally
2-3 days and the results are returned both as a text file
via email and as a color chromatogram printout.The DNA synthesis
service uses both an ABI 394 and an Expedite 8909. Together
they can produce between 30 and 50 oligos each day. Two
scales are available: 50nm and 200nm. Turnaround time is
often within 24 hours. Oligos are shipped out dried down
and quantitated and include a synthesis report. Cartridge
purification is available but for most applications it is
not necessary.The Peptide Synthesis Facility currently has
three ABI 431 Peptide Synthesizers employing FastMoc Chemistry.
We are capable of synthesizing 3-6 peptides per day depending
on their lengths at 2 different scales: 0.1mm and 0.25mm.
Turnaround time is usually between two to three weeks. All
peptides are returned with a Mass Spec analysis and an analytical
HPLC chromatogram with the exception of peptide libraries. |
| Tufts Center
for Vision Research
The Center was launched in May of 2000
with the establishment of a partnership agreement between
the New England Eye Center (Department of Ophthalmology)
and the New England Medical Center/Tufts University. The
Director of the TCVR, Dr. Elizabeth Fini, is a faculty member
of the CMDB graduate program.
The
mission of the Center is:
·
to
support research/educational activities and shared resources
for the 30 vision scientists on the Tufts campus,
·
to
foster collaborative interaction among Tufts vision scientists,
and between basic scientists and physician-scientists,
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to
attract well-trained people to do research on the visual
system and its diseases.
The
TCVR currently supports four core resource modules:
·
Transgenic
Mice:
This institutional facility is directed by Dr. Janis Lem
of the Department of Ophthalmology.
The department provides financial support for Dr.
Lem’s research and career development, and has also provided
much of the specialized equipment for the facility.
TCVR investigators have access to this facility along
with other Tufts investigators.
·
Morphology:
This module provides technical services, equipment resources,
and technical advice to TCVR investigators.
There are two components: histology/light microscopy
and confocal microscopy/electron microscopy.
The first component is housed in the 75 Kneeland
Street labs, the second component is part of the core facilities
of the Department of Anatomy and Cellular Biology, within
the Medical and Veterinary complex.
The directors of this module, Drs. Dale Hunter and
Judith West-Mays, are CMDB program faculty members.
·
Monoclonal
Antibodies and Tissue Resources: This module provides technical services, equipment resources, and technical
advice to TCVR investigators.
In addition, human eye tissues are provided to investigators
through the ophthalmology clinical services of the New England
Eye Center.
·
Computational
Biology Module: Recently, the rough draft of the human genome sequence was
completed. TCVR
investigators wish to take advantage of this wealth of new
information for their research.
This module provides a collaborative resource for
bioinformatics, data management, and biostatistics.
In addition, the module provides computer hardware
and software support, and computer resources.
The module is directed by Dr. Elizabeth Fini, a CMDB
program faculty member.
The module is staffed by computational geneticist
Dr. Kurt Wollenberg.
The module has a partnership arrangement with the
Center for Biostatistics Research, Division of Clinical
Care Research, Department of Medicine.
|
Program
in Cell, Molecular, and Developmental Biology
136 Harrison Avenue, 5th Floor
Boston, MA 02111
617-636-6685
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