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| Rob Willson, PhD
Dept. Anatomy and Cellular Biology
M&V 504
136 Harrison Avenue
Boston, MA 02111 |
Tel:
(617) 636-6607
Fax: (617) 636-6536
rwillson@emerald.tufts.edu |
| The Tufts
confocal Microscope Facility is located within the Department
of Anatomy and Cellular Biology and is in its fourth year
of operation. Services are provided for receiving living
or fixed samples labeled with 1-3 distinct fluorochromes
with the most popular application being 3D reconstructions
from optical sections, 2 or 3 overlay analysis for analyzing
spatial proximity and improved spatial resolution with
thick specimens. The fee-for-service facility provides
training, various image processing/analysis opportunities,
and hard copy output for publication or presentation purposes. |
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| Cathy Linsenmayer
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Tel:
(617) 636-0842
cathy.linsenmayer@tufts.edu
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Elizabeth Benecchi
Dept. Anatomy and Cellular Biology
136 Harrison Avenue
Boston, MA 02111 |
Tel: (617)
636-6929
lbenecch@opal.tufts.edu |
| The Electron
Microscope Applications Facility has become a university
resource. as evidenced by its utilization by the Departments
of Physiology, Microbiology, Neuroscience, NEMC Opthalmology,
Surgical Research, Nephrology, Gastroenterology, Chemistry
(Medford), the HNRC and MGH Surgery. It includes the Electron
Microscopy Laboratory (M&V 142) and a Microscopy
Suite (M&V 504) and as of 1999, has been directed
by the very capable and talented duo of Cathy Linsenmayer
and Liz Benecchi.
The Electron Microscope Laboratory
us equipped to prepare materials for conventional transmission
and scanning electron microscopy, and to provide such
specialized techniques as immunoelectron microscopy using
pre-embedding techniques and post-embedding approaches
using ultra thin cryo sections which current users and
staff routinely employ. The facility also supports molecular
structure analysis and epitope mapping of antibody binding
using electron microscopy of rotary shadowed and negatively
stained preparations, and a variety of photographic procedures
including printing of micrographs, production of plates
for publication and slide preparation.
This fee-for-service is open to researchers
in the Tufts Health Sciences Campus who wish to visualize
their samples at the sub-cellular level.The facility offers
support for fixation, embedding, sectioning, staining and
microscope use |
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| Rob
Willson, PhD
Dept. ANatomy and Cellular Biology
M&V 504
136 Harrison Avenue
Boston, MA 02111 |
Tel:
(617) 636-6607
Fax: (617) 636-6536
rwillson@emerald.tufts.edu |
| The
Tufts Microscopy & Digital Image Analysis Facility
is located within the Department of Anatomy and Cellular
Biology. Services are provided for imaging bright-field,
dark-field, phase-contrast, or fluorescently labeled samples.
Current equipment inclues a Zeiss Axioscope and a high-resolution
(1600 x 1200 pixels) Diagnostic Imaging Color Digital camera;
a CCD video camera and Image Intensifier is also available
for real-time biological imaging of live specimens. Quantitation
of images may be done using OPTIMAS image analysis software
which runs on a Pentium-III workstation. |
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| Michael Berne, PhD
Dept. Physiology
Stearns 808
136 Harrison Avenue
Boston, MA 02111 |
Tel:
(617) 636-2422
Fax: (617) 636-6737
michael.berne@tufts.edu |
| The Tufts DNA
Sequencing, DNA Synthesis, and Peptide Synthesis Facility
is located within the Department of Physiology. The
DNA sequencing lab consists of three automated ABI 37X DNA
sequencers. Each instrument runs 36 samples with a read
length of 500-800 bases. The turnaround time is generally
2-3 days and the results are returned both as a text file
via email and as a color chromatogram printout.The DNA synthesis
service uses both an ABI 394 and an Expedite 8909. Together
they can produce between 30 and 50 oligos each day. Two
scales are available: 50nm and 200nm. Turnaround time is
often within 24 hours. Oligos are shipped out dried down
and quantitated and include a synthesis report. Cartridge
purification is available but for most applications it is
not necessary.The Peptide Synthesis Facility currently has
three ABI 431 Peptide Synthesizers employing FastMoc Chemistry.
We are capable of synthesizing 3-6 peptides per day depending
on their lengths at 2 different scales: 0.1mm and 0.25mm.
Turnaround time is usually between two to three weeks. All
peptides are returned with a Mass Spec analysis and an analytical
HPLC chromatogram with the exception of peptide libraries. |
Program
in Cell, Molecular, and Developmental Biology
136 Harrison Avenue, 5th Floor
Boston, MA 02111
617-636-6685
Questions and comments to: Web
Development
Page last modified 10/21/08 |
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