T Cell Receptor Signaling in Live Cells
Tissue function depends on the ability of cells to
translate cues from the cellular microenvironment into biologically
significant actions. This requires cells to discriminate between signals
of differing streengths and qualities. DUring T cell activation, the
inability to respond correctly to external stimuli can result in either
uncontrolled infections or autoimmune disorders. My lab has a long-standing
interest in understanding the mechanisms which govern the response of
T cells. This interest extends to understanding the role of cellular
compartmentalization and signal transduction. For this purpose, I have
developed a system that allows visualization of events in live cells
and used this approach to study the molecular consequences of T cell
receptor (TCR)-induced signals. T cells that express fluorescently-tagged
chimeric proteins are placed on planar substrates, thereby permitting
direct assessment of complex events in real time. We have already used
this approach to examine TCR-induced cytoskeletal rearrangements and
to image the movements of five critical signaling intermediates in live
Jurkat T cells: ZAP-70, a TCR-associated tyrosine kinase, and the signaling
adapters, LAT, Grb2, Gads, and SLp-76. The picture below illustrates
our approach. To see movies and follow changes in real time, follow
this link.
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These images illustrate our technique. In the top row, Jurkat T cells expressing ZAP-70–EGFP were plated on coverslips. ZAP-70–EGFP and interference reflection microscopy images were collected every 15 s using the Zeiss LSM 410. The white arrow marks the earliest observed signaling cluster and contact point. In the center panel, Jurkat T cells expressing ZAP-70–EGFP were plated on stimulatory coverslips, fixed after 5 min, and stained for both TCR and phosphotyrosine. ZAP-70–EGFP, TCRζ, and phosphotyrosine were pseudocolored green, red, and blue, respectively. The TCR staining pattern shows a large cluster in the center of the contact; this strong signal results from the staining of a large, perinuclear pool of TCRζ. In the lower panel, cells were processed and imaged as in B after pretreatment and stimulation in the presence of 10 µM PP2, an inhibitor of Lck and Fyn. |
My lab is using this
and other techniques to approach four questions. First we would
like to know which proteins participate in TCR-induced signals.
We plan to dynamically image proteins such as Lyn, Fyn, PAG/Cbp,
Shc, Shb, Cbl, PI3K, PLCγ, ADAP, Itk and Vav as well as other
scaffolding proteins. FRET will also be used to determine the proximity
of various molecules when greater precision is needed. We are particularly
interested in SLP-76 and its potential role in signaling. |
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The
cartoon depicts possible interactions involving SLP-76 following
TCR signaling. A proposed role of the SH2 domain of SLP-76 is indicated
by the open box. |
A second and related question centers on how complexes are perturbed
when one of the components is missing. Cells deficient in selected
proteins because they are from knock-out mice or were treated with
RNAi will be used to approach this issue. We are also interested
in determining if critical signals originate in internalized vesicles.
Lastly, we are particularly interested in determining how the signals
and complex translocations we document are related to immune function
in vivo. These investigations should provide unique instights into
the interplay between signal transduction and cellular compartmentalizeion
during T cell activation. |
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