Anne Bishop, Ph.D.
Postdoctoral fellow
Ph.D. University College, London
Post Doc at Imperial College, London (2001-2005)
Thesis title:"Functional analysis of the Rac-Binding Protein POSH."

Publications
1. A Bishop, Baker S, Jenks S, Fookes M, O’Gaora P, Pickard D, Anjum M, Farrar J, Hien T, Ivens A and Dougan G. “Analysis of the hypervariable region of the Salmonella enterica genome associated with tRNAleuX ” – Journal of Bacteriology (2005) 187: 2469-2482

2. A Bishop, Dougan G and Baker S. “The Salmonella genome: a global view”, chapter within “Salmonella Infections: Clinical, Immunological and Molecular Aspects”, Cambridge University Press, editors P Mastroeni and D Maskell (2005) - In press book chapter

3. A Bishop and Hall A. “Rho GTPases and their effector proteins” –
Biochemical Journal (2000) 348:241-255, Review

Current research
In my current research I am investigating two aspects of infection with
the Vibrio cholerae bacterium: (1) The spatial control of
cyclic-di-guanylate signaling, which is a bacterial signaling network
that controls V. cholerae transistions between the host and external
environment; (2) Host innate immune responses to V. cholerae infection in the suckling mouse model.

Lori Bourassa
Graduate Student
B.S. Biology, Boston College

Current research
Investigation of Vibrio cholerae colonization of aqueous environmental hosts

Marc Kimball
Laboratory Manager
B. A. Ohio Wesleyan University

Julianna LeMieux
Graduate Student
B.S. in microbiology from Univ. of New Hampshire

Publications
1. Hava DL, LeMieux J, Camilli A. From nose to lung: the regulation behind Streptococcus pneumoniae virulence factors. Mol Microbiol. 2003 Nov;50(4):1103-10. PubMed

2. Gustincich S, Contini M, Gariboldi M, Puopolo M, Kadota K, Bono H, LeMieux J, Walsh P, Carninci P, Hayashizaki Y, Okazaki Y, Raviola E. Gene discovery in genetically labeled single dopaminergic neurons of the retina. Proc Natl Acad Sci U S A. 2004 Mar 26. PubMed

3. Gustincich S, Batalov S, Beisel KW, Bono H, Carninci P, Fletcher CF, Grimmond S, Hirokawa N, Jarvis ED, Jegla T, Kawasawa Y, LeMieux J, Miki H, Raviola E, Teasdale RD, Tominaga N, Yagi K, Zimmer A, Hayashizaki Y, Okazaki Y; RIKEN GER Group; GSL Members. Analysis of the mouse transcriptome for genes involved in the function of the nervous system. Genome Res. 2003 Jun;13(6B):1395-401. PubMed

Current research
Study of zinc metalloproteases of Streptococcus pneumoniae and roles in infection.

Jane Liu, Ph.D.
Postdoctoral fellow
Ph.D. Harvard University, Cambridge, MA
Thesis title: "Functional dissection of natural RNAs by nonhomologous
random recombination and in vivo selections."

Publications
1. Liu, J. M.; Bittker, J. A.; Lonshteyn, M.; Liu, D. R. (2005)
"Functional Dissection of sRNA Translational Regulators Using
Nonhomologous Random Recombination and In Vivo Selection" Chem. Biol.
12, 757-767. Pubmed

2. Bittker, J. A.; Le, B. V.; Liu, J. M.; Liu, D. R. (2004) "Directed
Evolution of Protein Enzymes Using Nonhomologous Random Recombination"
Proc. Natl. Acad. Sci. USA 101, 7011-7016. Pubmed

3. Paley, R. S.; Liu, J. M.; Lichtenstein, B. R.; Knoedler, V. L.;
Sanan, T. T.; Adams, D. J.; Fernandez, J.; Rablen, P. R. (2003)
"Simultaneous and Stereoselective Formation of Planar and Axial
Chiralities in Enantiopure Sulfinyl Iron Diene Complexes" Org. Lett.
5, 309-312. Pubmed

Current research
In my current research I am investigating small non-coding RNAs
(sRNAs) that are involved in mediating the transition of Vibrio
cholerae from the environment to infection of the host small
intestine.

Hector Martinez-Wilson
Graduate Student in combined M.D./Ph.D. program
B.S., Tufts University, Medford, MA

Publications
1. Harris, M. T., K. Lai, et al. (2000). "Chitin synthase in the filarial parasite, Brugia malayi." Mol Biochem Parasitol 111(2): 351-62.

2. Sereti I, Martinez-Wilson H, Metcalf JA, Baseler MW, Hallahan CW, Hahn B, Hengel RL, Davey RT, Kovacs JA, Lane HC. Long-term effects of intermittent interleukin 2 therapy in patients with HIV infection: characterization of a novel subset of CD4(+)/CD25(+) T cells. Blood. 2002 Sep 15;100(6):2159-67. PubMed

3. Sereti, I., K. B. Anthony, et al. (2004). "IL-2-induced CD4+ T-cell expansion in HIV-infected patients is associated with long-term decreases in T-cell proliferation." Blood 104(3): 775-80.

4. Osorio, C. G., H. Martinez-Wilson, et al. (2004). "The ompU Paralogue vca1008 is required for virulence of Vibrio cholerae." J Bacteriol 186(15): 5167-71.

5. Osorio, C. G., J. A. Crawford, et al. (2005). "Second-generation recombination-based in vivo expression technology for large-scale screening for Vibrio cholerae genes induced during infection of the mouse small intestine." Infect Immun 73(2): 972-80.

Current research
Study of Vibrio cholerae gene regulation during infection.

Ernesto J. Muñoz Elías, Ph.D.
Postdoctoral Fellow
Ph.D., Rockefeller University, New York, NY
Thesis title: “The Role of the Glyoxylate Cycle in the Pathogenesis of Mycobacterium tuberculosis.”

Publications
1. Muñoz-Elías E. J., and J. D. McKinney JD.  Mycobacterium tuberculosis isocitrate lyases 1 and 2 are jointly required for in vivo growth and virulence.  Nat Med. 2005 Jun;11(6):638-44. Epub 2005 May 15.

2. Muñoz-Elías E. J., J. Timm, T. Botha, W. T. Chan, J. E. Gomez, and J. D. McKinney.  Replication dynamics of Mycobacterium tuberculosis in chronically infected mice.  Infect Immun. 2005 Jan;73(1):546-51.

3. Muñoz-Elías, E. J. and J.D. McKinney (2002).  Bacterial Persistence: strategies for survival. In: Immunology of Infectious Diseases, Ed. Kaufmann, S., Sher, A. & Ahmed, R. ASM Press.

4. McKinney J. D., K. Honer zu Bentrup, E. J. Muñoz-Elías, A. Miczak, B. Chen, W. T. Chan, D. Swenson, J. C. Sacchettini, W. R. Jacobs Jr, and D. G. Russell.  Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyase.  Nature. 2000 Aug 17;406(6797):735-8. 

Current research
Investigation of colonization properties and virulence mechanisms of Streptococcus pneumoniae.

Eric Nelson
Graduate Student in combined M.D./Ph.D. program
B.A. Cornell University, Ithaca, NY
M.S. University of Hawaii, Honolulu, HI.

Publications

1. Nelson, E.J., Chowdhury A., Harris J.B., Begum Y. A., Chowdhury F., Khan A. I., Larocque R.C. Bishop A. L., Ryan, E. T., Camilli A. , Qadri F., Calderwood, S.B. Complexity of rice-water stool from patients with Vibrio cholerae plays a role in the transmission of infectious diarrhea. Proc. Natl. Acad. Sci. USA. 2007, Nov. 27; 104(48): 19091-6.

2. Schild, S., Tamayo R., Nelson E.J., Qadri F. , Calderwood S.B., Camilli A. Genes induced late in infection increase fitness of Vibrio cholerae after release into the environment. Cell Host Microbe. 2007, Oct. 11;2(4): 264-77.

3. Nelson E.J., Tunsio H.S., Fidopiastsis P.M., Sorum H, Ruby E.G. A novel lux operon in the cryptically bioluminescent fish pathogen Vibrio salmonicida is associated with virulence. Appl. Environ. Microbiol. 2007, Mar.; 73)6): 1825-33.

4. Butler S.M., Nelson E.J., Chowdhury N., Faruque S.M., Calderwood S.B., Camilli A. Cholera stool bacteria repress chemotaxix to increase infectivity. Mol. Microbiol. 2006 Apr; 60(2):417-26.

4. E. J. Nelson and W. C. Ghiorse. Isolation and identification of Pseudoalteromonas piscicida strain Cura-d associated with diseased
damselfish (Pomacentridae) eggs. J of Fish Diseases. 1999 (22). 253-260.

Current research
Study of Vibrio cholerae transmission and pathogenicity.

Jason Pratt
Graduate Student
B.S. in Biology, Wake Forest University

Publications
1. Davis, B.M., M. Quinones, J. Pratt, Y. Ding, and M.K. Waldor. 2005. Characterization of the sRNA RyhB and its regulon in Vibrio cholerae. J. Bacteriol. 2005 Jun;187(12):4005-14.

Current research
Mechanism of gene regulation by cyclic diguanylate (c-di-GMP).

Katherine Price
Graduate Student
B.S. in Genetics and Microbiology from Univ. of Georgia

Current research
Characterization of the subcellular localization of the Streptococcus
pneumoniae cytotoxin pneumolysin.

Rita Tamayo, Ph.D.
Postdoctoral Fellow
Ph.D., University of Texas Health Science Center, San Antonio, TX
Thesis Title: PmrA-mediated lipopolysaccharide substitutions: Role in antimicrobial peptide resistance and virulence of Salmonella enterica serovar Typhimurium

Publications
1. Tamayo, R., Ryan, S. R., McCoy, A. J. and John S. Gunn. Identification and genetic characterization of PmrA-regulated genes and genes involved in polymyxin B resistance in Salmonella enterica serovar Typhimurium. Infection and Immunity. 2002; 70:6770-6778. Pubmed

2. Tamayo, R., Portillo, A. C. and J. S. Gunn. Mechanisms of Bacterial Resistance to Antimicrobial Peptides. Mammalian Antimicrobial Peptides, Advances in Molecular and Cellular Microbiology Series. Eds. D. A. Devine and R. E. W. Hancock. Cambridge University Press. 2004; 323-348.

3. Tamayo, R., Prouty, A.M. and J.S. Gunn. Identification and functional analysis of Salmonella enterica serovar Typhimurium PmrA-regulated genes. FEMS Immunology and Medical Microbiology. 2005; 43(2): 249-58. Pubmed

4. Tamayo, R, Choudhury, B., Septer, A., Merighi, M., Carlson, R. and J. S. Gunn. Identification of cptA, a PmrA-regulated locus required for phosphoethanolamine modification of the Salmonella enterica serovar Typhimurium lipopolysaccharide core. Journal of Bacteriology. 2005 May;187(10):3391-9.

5. Tamayo, R., A. D. Tischler, and A. Camilli.  The EAL domain protein VieA is a cyclic diguanylate phosphodiesterase.  J Biol Chem. 2005 Sep 30;280(39):33324-30. Epub 2005 Aug 4.

Current Research
Study of cyclic diguanylate EAL phosphodiesterases in Vibrio cholerae.

Tim van Opijnen, Ph.D.
Postdoctoral fellow
Ph.D. University of Amsterdam
Thesis title Virus and environment: How HIV-1 replication and evolution are driven by the host environment

Publications:
1. van Opijnen T, deRonde A, Boerlijst MC, Berkhout B. Adaptation of HIV-1 depends on the host-cell environment. (in press)

2. van Opijnen T, Boerlijst MC, Berkhout B. 2006. Effects of random mutations in the human immunodeficiency virus type 1 transcriptional promoter on viral fitness in different host cell environments Journal of Virology 80 (13): 6678-6685

3. van Opijnen T, Berkhout B 2005. The host environment drives HIV-1 fitness Reviews in medical virology 15 (4): 219-233

4. van Opijnen T, Jeeninga RE, Boerlijst MC, Pollakis GP, Zetterberg V, Salminen M, Berkhout B 2004. Human immunodeficiency virus type 1 subtypes have a distinct long terminal repeat that determines the replication rate in a host-cell-specific manner Journal of virology 78 (7): 3675-3683

Current research
Identify host genetic factors that drive resistance to Streptococcus pneumoniae.