Analysis of Bacterial Uptake & Growth within Mammalian Cells


Principal Investigator
Ralph Isberg
Professor
Howard Hughes Medical Institute Investigator

Program Affiliations
Molecular Microbiology

Contact Information
Department of Microbiology
Tufts University
136 Harrison Avenue
Boston, MA 02111

Office (617) 636-3993
Lab (617) 636-4092
Fax (617) 636-0337
Email  
Ralph.Isberg@tufts.edu

  1. Heidtman, M.I., Chen, E, Moy, M-Y. and Isberg, R.R. 2009. Large scale identification of Legionella pneumophila Dot/Icm substrates that modulate host cell vesicle trafficking pathways. Cell. Microbiol.

  2. Losick, V.P., Stephan, K, Smirnova, I.I. Isberg, R.R. and Poltorak, A. 2009.  A hemidominant Naip5 allele causes restriction of growth within macrophages from the wild-derived MOLF/Ei mouse.  Infect. Immun. 77:196-204.

  3. Wong, K-W., Mohammadi, S. and Isberg, R.R. 2008. The polybasic region of Rac1 modulates bacterial uptake independently of self-association and membrane targeting.   J. Biol. Chem. 283:35954-65. (Link to article)

  4. Liu, M., Conover, G.M., and Isberg, R.R. 2008. Legionella pneumophila EnhC: an envelope protein required for intracellular growth in the presence of cytokine stimulation. Cell Microbiol. 10: 1906-1923. (Link to article)

  5. Conover, G.M., Martinez-Morales, F., Heidtman, M.I., Luo, Z.Q., Tang, M., Chen, C., Geiger, O., and Isberg, R.R. 2008. Phosphatidylcholine synthesis is required for optimal function of Legionella pneumophila virulence determinants. Cell Microbiol. 10:514-528. (Link to article)

  6. Machner, M.P., and Isberg, R.R. 2007. A bifunctional bacterial protein links GDI displacement to Rab1 activation. Science. 318:974-977.

  7. Auerbuch, V., and Isberg, R.R. 2007. Growth of Yersinia pseudotuberculosis in mice occurs independently of Toll-like receptor 2 expression and induction of interleukin-10. Infect Immun. 75:3561-3570. (Link to article)

  8. Laguna, R.K., Creasey, E.A., Li, Z., Valtz, N. and Isberg, R.R. 2006. A Legionella pneumophila-translocated substrate that is required for growth within macrophages and protection from host cell death. Proc Nat Acad Sci U S A. 103:18745-18750. (Link to article)

  9. Wong, K.W., Mohammadi, S. and Isberg, R.R. 2006. Disruption of RhoGDI and RhoA regulation by a Rac1 specificity switch mutant. J. Biol. Chem, 281:40379-40388.

  10. Losick, V.P. and Isberg, R.R. 2006. NF-kB translocation prevents host cell death after low-dose challenge by Legionella pneumophila. J. Exp. Med. 203:2177-2189. (Link to article)

  11. Barnes, P.D., Bergman, M.A., Mecsas, J. and Isberg, R.R. 2006. Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine. J. Exp. Med. 203:1591-1601. (Link to article)

  12. Machner, M. and Isberg, R.R. 2006. Targeting of host Rab GTPase function by the intravacuolar pathogen Legionella  pneumophila.  Dev. Cell. 11:47-56.

  13. Dorer, M.S., Kirton, D., Bader, J.S., and Isberg R.R. 2006. RNA interference analysis of Legionella in Drosophila cells: exploitation of early secretory apparatus dynamics.  PLoS Pathog. 2:315-327. (Link to article)

  14. Wong, K.W. and Isberg, R.R. 2005. Yersinia pseudotuberculosis spatially controls activation and misregulation of host cell Rac1. PLoS Pathog. 1:125-136. (Link to article)

  15. Derré, I. and Isberg, R.R. 2005. LidA, a tranlocated substrate of Legionella pneumophila type IV secretion system, interferes with the early secretory pathway. Infect. Immun. 73:4370-4380. (Link to article)

  16. Li, Z., Solomon, J.M. and Isberg, R.R. 2005. Dictyostelium discoideum strains lacking the RtoA protein are defective for maturation of the Legionella pneumophila replication vacuole. Cell. Microbiol. 7: 431-442.

  17. Derré, I. and Isberg, R.R. 2004. Legionella pneumophila replication vacuole formation involves rapid recruitment of proteins of the early secretory system. Infect. Immun. 72:3048-3053. (Link to article)

  18. Luo, Z.Q. and Isberg, R.R. 2004. Multiple substrates of the Legionella pneumophila Dot/Icm system identified by interbacterial protein transfer. Proc. Nat Acad. Sci, USA. 101:841-846. (Link to article)

  19. Wong, K.W. and Isberg, R.R. 2003. Arf6 and phosphoinositol-4-phosphate-5-kinase activities permit bypass of the Rac1 requirement for beta1 integrin-mediated bacterial uptake. J. Exp. Med. 198:603-614. (Link to article)

  20. Conover, G.M., Derré, I., Vogel, J.P., and Isberg, R.R. 2003. The Legionella pneumophila LidA protein: a translocated substrate of the Dot/Icm system associated with maintenance of bacterial integrity. Mol. Microbiol. 48:305-322.