Principal Investigator
Ralph Isberg
Professor
Howard Hughes Medical Institute Investigator
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Program Affiliations
Molecular Microbiology
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Contact Information
Department of Microbiology
Tufts University
136 Harrison Avenue
Boston, MA 02111
Office (617) 636-3993
Lab (617) 636-4092
Fax (617) 636-0337
Send Email |
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Selected Publications
Machner, M. and Isberg, R.R. (2007). A Bifunctional Region in a Bacterial Protein Links GDI Displacement to Rab1 activation. Science, in press.
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Conover,C. Martinez-Morales, R., Heidtman,M.I., Luo, Z.-Q., Tang,M., Chen,C., Geiger, O and Isberg,R.R. (2007). Phosphatidylcholine synthesis is required for optimal function of Legionella pneumophila virulence determinants. Cell. Microbiol, in press.
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O’Connor, T., Heidtman, M. and Isberg, R.R. (2007). Mechanisms of Intracellular Survival and Replication of Legionella pneumophila. In Legionella: Molecular Microbiology. Horizons Press, NY.
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Auerbuch, V. and Isberg, R.R. (2007). Growth of enteropathogenic Yersinia in mice occurs independently of Toll-like receptor 2 expression and induction of interleukin 10. Infect. Immun., 70: 3561-3570.
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Wong, K.W., Mohammadi, S. and Isberg, R.R. (2006). Disruption of RhoGDI and RhoA regulation by a Rac1 specificity switch mutant. J. Biol. Chem, 281:4 0379-88.
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Laguna, R.K., Creasey, E.A., Li, Z., Valtz, N. and Isberg, R.R. (2006). A Legionella pneumophila translocated substrate that is required for growth within macrophages and protection from host cell death. Proc Nat Acad Sci U S A. 103: 18745-18750.
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Losick, V.P and Isberg, R.R. (2006). NF-kB prevents premature host cell death after low dose challenge by Legeionella pneumophila. J. Exp. Med. 203:2177-89.
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Williams, R.S.B., Boeckeler, K., Gräf, R. Müller-Taubenberger, Li, Z., Isberg, R.R., Wessels, D., Soll, D.R., Alexander, H., Alexander, S. (2006). Towards a molecular understanding of diseases using Dictyostelium discoideum. Trends Mol. Medicine. 12: 415-24.
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Barnes, P. , Bergman, M, Mecsas, J. and Isberg, R.R. (2006). Yersinia pseudotuberculosis disseminates directly from a replicating bacterial pool in the intestine. J. Exp. Med. 203: 1591-1601.
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Machner, M. and Isberg, R.R. (2006). Machner, M. and Isberg, R.R. (2006). Targeting of host Rab GTPase function by the intravacuolar pathogen Legionella pneumophila. Dev. Cell. 11: 47-56.. Dev. Cell. 11: 47-56.
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VanRheenen, S.M., Luo, Z.-Q., O’Connor, T. and Isberg, R.R. (2006). Members of a Legionella pneumophila family of proteins with ExoU/phospholipase A active sites are translocated to target cells. Infect. Immun. 74: 3597-3606.
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Dorer, M.S. and Isberg, R.R. (2006).
Non-vertebrate hosts in the analysis of host-pathogen
interactions. Microbes. Immun., In press
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Wong, K.-W. and Isberg. 2005. Emerging
views of on integrin signaling via Rac1 during invasin-promoted
bacterial uptake. Curr. Op. Microbiol. 8: 4-9.
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Buscher, B.A., Conover
, G.M., Miller, J.L., Meyers, S.N., Isberg, R.R. and Vogel, J.P. 2005. The Legionella
pneumophila DotL protein, a member of the TraG-coupling
protein family, is essential for viability. J. Bacteriol.,
in press.
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Derré, and Isberg , R.R. 2005.
LidA, a tranlocated substrate of Legionella type IV secretion
system, interferes with the early secretory pathway.
Infect. Immun. In press.
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Wong, K.-W. and Isberg, R.R. 2005. Yersinia
pseudotuberculosis spatially controls activation
and misegulation of host cell Rac1. PLoS Pathogens
Volume 1 In Press.
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Li, Z., Solomon, J.M. and Isberg,
R.R. 2005. Dictyostelium discoideum strains
lacking the RtoA protein are defective for maturation
of the Legionella pneumophila replication vacuole.
Cell. Microbiol. 7: 431-442.
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Wong, K.-W., Barnes, P. and Isberg,
R.R. 2004. The Invasin Protein of Enteropathogenic Yersinia Species:
Integrin Binding and Role in Gastrointestinal Diseases.
In “Molecular and Cellular Biology of Pathogenic Yersinia,” Horizon
Press (Norfolk, UK), in press.
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Derré, I. and R.R. Isberg. 2004. Legionella
pneumophila replication vacuole formation involves
rapid recruitment of proteins of the early secretory
system. Infect Immun. 72:3048-3053.
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Derré, I. and Isberg, R.R. 2004. Macrophages
from mice having the restrictive Lgn1 allele exhibit
multifactorial resistance to Legionella pneumophila.
Infect. Immun. 72: 6221-6229.
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VanRheenen, S.M and Isberg,
R.R. 2004. DotU and IcmF are required for
optimal effector translocation and trafficking of the Legionella
pneumophila vacuole. Infect. Immun. 72(10):5972-82.
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Derré, I. and Isberg,
R.R. 2004. Macrophages from mice having the
restrictive Lgn1 allele exhibit multifactorial
resistance to Legionella pneumophila. Infect.
Immun. Infect Immun. 2004 Nov; 72(11):6221-9.
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Derré, I. and Isberg,
R.R. 2004. Legionella pneumophila replication
vacuole formation involves rapid recruitment of proteins
of the early secretory system. Infect. Immun. 72:
3048-3053.
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Luo, Z-Q. and Isberg, R.R. 2004.
Multiple Novel Substrates of the Legionella pneumophila Dot/Icm
system Identified by Interbacterial Protein Transfer.
Proc. Nat Acad. Sci, USA. 101: 841-846.
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Duménil, G., Montminy, T. ,
Tang, M. and Isberg, R.R. 2004. Membrane
insertion and efflux promoted by the Legionella pneumophila IcmQ
protein. J. Biol. Chem. 279:4686-95.
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Isberg, R.R., Barnes,
P. and Wong, K. 2003. Uptake of Yersinia pseudotuberculosis
into cultured cells and integrin receptor signalling.
In Microbial subversion of host cells Editors C. D. O’Connor,
D. G. E. Smith. Cambridge University Press.
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Wong, K.-W. and Isberg, R.R. 2003.
Arf6 and Phosphoinositol-4-Phosphate-5-Kinase Activities
Allow Bypass of the Rac1 Requirement for beta1 Integrin-Mediated
Bacterial Uptake. J. Exp. Med., 198: 603-614.
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Solomon, J., Leung, G., and Isberg,
R.R. 2003. Intracellular replication of Mycobacterium
marinum within D. discoideum: efficient replication
in the absence of host coronin. Infect. Immun. 71:
3578-86
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Conover, G., Derré, I., Vogel,
J, and Isberg, R.R. 2003. The Legionella
pneumophila LidA protein: a translocated substrate
of the Dot/Icm system associated with maintenance of
bacterial integrity..Mol. Microbiol. 48: 305-322.
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Isberg, R.R. and
Barnes, P. 2002. Dancing with the host: flow-dependent
bacterial adhesion. Cell. 110: 1-4. PubMed
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Rankin, S, Li, Z and Isberg,
R.R. 2002. Macrophage–induced Genes
of Legionella pneumophila: Protection from
Reactive Intermediates and Solute Imbalance During
Intracellular Growth. Infect. Immun. 70: 3637-3648. PubMed
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Isberg, R.R. 2002.
Invasin. In The Prokaryotes, Martin Dworkin and Stanley
Falkow, eds. Springer-Verlag, NY.
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Srivastava, A. and Isberg,
R.R. 2002. Uptake of Yersinia by
cultured cells. In “Molecular Cellular Microbiology,” P.
Sansonetti and A. Zychlinsky, eds. Academic Press,
NY.
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Isberg, R.R. and
Duménil, G. 2001. Delivering dangerous cargoes.
Nature Struct. Biol. 8: 1006-1008.
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Watarai, M., Kirby, J., Growney, J.D.,
Dietrich, W.F., and Isberg, R.R. 2001. Legionella
pneumophila is internalized by a macropinocytotic
uptake pathway controlled by the Dot/Icm system and the
mouse Lgn1 locus. J. Exp. Med. 194: 1081-96. PubMed
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Alrutz, M.A., Srivastava, A., Wong,
K., Tran Van Nhieu, G., D’souza-Schorey, C., Tang,
M., Snapper, S. and Isberg, R.R. 2001.
Efficient uptake of Yersinia pseudotuberculosis via
integrin receptors involves a Rac1-Arp2/3 pathway that
bypasses N-WASP function. Mol Microbiol., 43:
689-703. PubMed
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Werner, E., Kheradmand, F., Isberg,
R.R. and Werb, Z. 2001. Phagocytosis mediated
by Yersinia invasin induces collagenase-1
expression in rabbit synovial fibroblasts through a
proinflammatory cascade. J. Cell. Sci 114: 3333-3343.
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Duménil, G. and Isberg,
R.R. 2001. The Legionella pneumophila IcmR
protein exhibits chaperone activity for IcmQ by preventing
its participation in high molecular weight complexes.
Mol. Microbiol.. 40: 1113-1128. PubMed
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Isberg, R.R. and
Barnes, P. 2001. Subversion of integrin receptors by Yersinia.
J. Cell. Sci., 114: 21-28
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Watarai, M., Andrews, H.L. and Isberg,
R.R. 2001. Formation of a fibrous structure on the surface
of Legionella pneumophila associated with exposure
of DotO and DotH proteins after intracellular growth.
Mol Microbiol. 39: 313-330.
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Solomon, J. and Isberg, R.R. 2000.
Dictyostelium discoideum and the analysis of intracellular
pathogens. Trend. Microbiol. 8: 478-480
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Krukonis, E.S. and Isberg,
R.R. 2000. Integrin beta1-Chain
Residues Involved in Substrate Recognition and Specificity
of Binding to Invasin . Cellular Microbiology. 2:
219-230. PubMed
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Isberg, R.R. and
Barnes, P. 2000. Border patrols and secret passageways
across the intestinal epithelium. Trend. Microbiol. 8:
291-293.
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Isberg, R.R. 2000.
Signaling and invasin-promoted uptake via integrin receptors.
Microbes. Infect. 2: 793-801.
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Solomon, J., Cardelli, J. and Isberg,
R.R. 2000. Dictyostelium discoideum as a model
host system for the analysis of Legionella pneumophila virulence.
Infect. Immun. 68: 2939-2947.
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Dersch, P. and Isberg, R.R. 2000.
An immunoglobulin superfamily-like domain unique to the Yersinia pseudotuberculosis
invasin protein is required for stimulation of bacterial
uptake via integrin receptors. Infect. Immun. 68:
2930-2938.
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Isberg, R.R. 2000.
Identification and Analysis of Proteins Expressed by
Bacterial Pathogens in Response to Host Tissues. In Bacterial
Stress Response, pp. 289-304. G. Storz and R. Hengge-Aronis,
eds. ASM Press, Washington, D.C.
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Hamburger, Z.A., Brown, M.S., Isberg,
R.R., Bjorkman, P.J. (1999). Crystal Structure
of Invasin: A Bacterial Integrin-Binding Protein. Science. 286:
291-295. PubMed
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Dersch, P. and Isberg, R.R. 1999.
A region of the Yersinia pseudotuberculosis
invasin protein enhances integrin-mediated uptake into
mammalian cells and promotes self-association. EMBO J. 18:
1199-1213. PubMed
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Vogel, J. and Isberg, R.R. 1999.
Cell biology of Legionella pneumophila Cur Opinion
Biol. 2: 30-34.
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Alrutz, M.A. and Isberg, R.R. 1998.
Involvement of focal adhesion kinase in invasin-mediated
uptake. Proc. Nat. Acad. Sci., USA, 95: 13658-13663. PubMed
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Krukonis, E.S., Dersch, P., Eble,
J.A. and Isberg, R.R. 1998. Differential
effects of integrin alpha chain mutations on invasin
and natural ligand interaction. J. Biol. Chem, 273:
31837-31843.
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Eble, J.A., Wucherpfennig, K.W., Gauthier,
L., Dersch, P., Krukonis, E., Isberg, R.R.,
Hemler, M.E. 1998. Recombinant soluble human alpha3beta1integrin:
purification, processing, regulation and specific binding
to laminin-5 and invasin in a mutually exclusive manner.
Biochemistry, 37: 10945-10955.
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Kirby, J.E. and Isberg, R.R. 1998.
Legionnaire’s disease: The pore macrophage and
the legion of terror within. Trends in Microbiology, 6:
256-258.
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Roy, C.R., Berger, K. and Isberg,
R.R. 1998. Legionella pneumophila DotA
protein is required for early phagosome trafficking
decisions that occur within minutes of bacterial uptake.
Mol. Microbiol, 28: 633-644. PubMed
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Krukonis, E.S. and Isberg,
R.R. 1998. SWIM analysis allows rapid identification
of residues involved in invasin-mediated bacterial
uptake. Gene, 211: 109-116.
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Andrews, H.L., Vogel, J.P., and Isberg,
R.R. 1998. Identification of linked Legionella
pneumophila genes essential for intracellular
growth and evasion of the endocytic pathway. Infect.
Immun., 66: 950-958.
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Vogel, J.P, Andrews, H.L., Wong, S.K.,
and Isberg, R.R. 1998. Conjugative transfer
by the virulence system of Legionella pneumophila.
Science, 279: 873-876. PubMed
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| Kirby, J.E., Vogel, J.P., Andrews,
H.L. and Isberg, R.R. 1998. Evidence
for pore formation by Legionella pneumophila.
Mol. Microbiol., 27: 323-336. |
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