Messenger RNA Processing

Jason Kuehner
Posdoctoral Associate
Ph.D., University of Wisconsin-Madison, Cellular and Molecular Biology
B.A., Cornell College, Biochemistry and Molecular Biology

Thesis Topic
Mechanism and regulation of yeast RNA polymerase II transcription initiation and termination.

Current Research
I am interested in the role of the cleavage/polyadenylation machinery in poly(A)-dependent transcription termination. I'm currently studying the molecular events that lead to termination as well as attempting to identify new factors involved in this fundamental process of gene expression.

Publications

1. Cloud-Hansen, K.A., Kuehner, J.N., Tong L., Miller, S., and Handelsman, J. (2008). Money, sex, and drugs: a case study to teach the genetics of antibiotic resistance.  CBE Life Sci. Educ. 7(3): 302-309.
2. Kuehner, J.N. and Brow, D.A. (2008). Regulation of a eukaryotic gene by GTP-dependent start site selection and transcription attenuation. Mol. Cell. 31(2): 201-211.
3. Steinmetz, E.J., Warren C.L., Kuehner, J.N., Panbehi, B., Ansari, A.Z., and Brow, D.A. (2006). Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase. Mol. Cell. 24(5): 735-746.
4. Kuehner, J.N., and Brow, D.A. (2006). Quantitative analysis of in vivo initiator selection by yeast RNA polymerase II supports a scanning model. J. Biol. Chem. 281 (20): 14119-14128.