Bacillus subtilis & Clostridium difficile

Shaun Brinsmade
Postdoctoral Associate
Ph.D. University of Wisconsin-Madison
B.S. University of Connecticut-Storrs

Thesis Topic
Genetics and Biochemistry of Class II Phosphotransacetylases: Enzymes and Sensors of Nutrition, Energy and Redox State of Prokaryotic Cells.

Current Research
Integrative systems biology approaches to investigate and to understand genetic programs of adaptation in gram-positive bacteria.

Publications

1. Brinsmade SR, Escalante-Semerena JC. In vivo and in vitro analyses of single-amino acid variants of the Salmonella enterica phosphotransacetylase enzyme provide insights into the function of its N-terminal domain. J Biol Chem. 2007 Apr 27;282(17):12629-12640. Epub 2007 Mar 5.
2. Kim YR, Brinsmade SR, Yang Z, Escalante-Semerena JC, Fierer J. Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice. Infect Immun. 2006 Apr;74(4):2498-2502.
3. Brinsmade SR, Paldon T, Escalante-Semerena JC. Minimal functions and physiological conditions required for growth of Salmonella enterica on ethanolamine in the absence of the metabolosome. J Bacteriol. 2005 Dec;187(23):8039-8046.
4. Brinsmade SR, Escalante-Semerena JC. The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity. J Bacteriol. 2004 Mar;186(6):1890-1892.
5. Silvaggi NR, Anderson JW, Brinsmade SR, Pratt RF, Kelly JA. The crystal structure of phosphonate-inhibited D-Ala-D-Ala peptidase reveals an analogue of a tetrahedral transition state. Biochemistry. 2003 Feb 11;42(5):1199-1208.