The Tufts University/Tufts Medical Center Imaging Facility (TIF) provides equipment and expertise for confocal and TIRF (total internal reflection fluorescence) microscopy. The facility includes a Leica TCS SP2 AOBS and a Leica TCS SPE confocal microscope. For researchers interested in live cell imaging, both confocal systems as well as the TIRF are equipped with incubators. The new Nikon confocal and TIRF systems have Perfect Focus which prevents focus drift during imaging. For the study of dynamic processes, the Nikon confocal has a resonant scanner capable of imaging 30 fps (512 x 512 pixel field). We also have access to the Perkin-Elmer spinning disk confocal (Ultraview LCI) operated by the Bunnell Laboratory, in association with the SCIID Core Facility and the Department of Pathology. The TIF is maintained with support from the Tufts Center for Neuroscience Research. We provide imaging equipment including FRAP, FRET and the use of photoactivatible GFP, to all scientists of Tufts University and Tufts Medical Center.
We also offer equipment and expertise for laser capture microdissection, using an Arcturus PixCell IIe instrument. Please see the TIF equipment link for additional information. A protocol for LCM use, kindly provided by Dr. Maribel Rios, is available here.
The CNR offers access to a Zeiss Axioplan microscope located in Arnold 309. This instrument is configured for fluorescence microscopy, brightfield and differential interference contrast (DIC) imaging, and has image acquisition capabilities.
The TIF is supervised by Dr. Rob Jackson, and is managed on a day-to-day basis by Dr. Alenka Lovy. Dr. Lovy provides training on confocal microscopy, TIRF (total internal reflection fluoerescence) microscopy, as well as laser capture microdissection. Dr. Fanny Ng (Shui-Ying.Ng@tufts.edu) provides training on the Zeiss Axioplan microscope in Arnold 309. Dr. Lovy can be reached at Alenka.Lovy@tufts.edu or by phone at 617-636-3795. Our facility is located in Stearns 207, adjacent to the Stearns elevator.
Spinning disk confocal microscopy and deconvolution microscopy are available through the labs of Drs. Stephen Bunnell (Pathology) and Robbie O'Connor (Infectious Disease). Please contact Dr. Lovy for information about access to these microscopes.
Please follow the links below for additional information about the TIF.