Education | Laboratory Personnel | General Research Interest | Selected Research Projects
Research and Clinical Interests | Major Specialized Equipment Items Available | Selected Publications
TCSVM Faculty

Mohammed S. Anwer
Distinguished Professor
Associate Dean for Research
Department of Biomedical Sciences
Pharmacology
Phone: 508-839-8788
Fax: 508-839-8787
Email: sawkat.anwer@tufts.edu

Education
DMVH - Munich University - 1980
PhD - Kansas State University - 1973
MS - Dhaka University - 1968

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Laboratory Personnel
Holly Jameson, Senior Research Technician
Christopher Schonhoff, Ph.D., Research Assistant Professor

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General Research Interest
Functional abnormalities associated with various liver diseases lead to accumulation of toxic products (such as bilirubin in jaundice and bile acids) in blood and premature death of liver cells. One of the major determinants is the inability of the liver to properly regulate bile acid transport from blood to bile. Studies are conducted to better understand the mechanisms regulating bile acid transport. Specifically, studies are designed to determine the role of various kinases and phosphatases in the regulation of bile acid transport. Techniques used are cell culture, transfection of cell lines, solute uptake, western blots, protein phosphorylation, etc.

Research Sponsor Interest:

  • Privately Funded Research
  • Federally Funded Research
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Selected Research Projects

  1. McConkey, M., Gillin, H., Webster, C.R.L., Anwer, M.S.:  Cross-talk between PKCζ and PKB in cAMP mediated TC uptake and Ntcp translocation in hepatocytes.  J. Biol. Chem.  279:20882-20888, 2004.
  2. Anwer, M.S.:  Cellular regulation of hepatic bile acid transport in health and cholestasis.  Hepatology  39:581-590, 2004.
  3. Anwer, M.S., Gillin, H., Mukhopadhaya, S., Balasubramaniyan, N., Suchy, F.J., Ananthanarayanan, M.:  Dephosphorylation of Ser-226 facilitates plasma membrane retention of Ntcp.  J. Biol. Chem. 280:33687-33692, 2005.
  4. Sarkar, S., Bananis, E., Nath, S., Anwer, M.S., Wolkoff, A.W., Murray, J.M.:  PKCζ is required for microtubule-based motility of vesicles containing the Ntcp transport.  Traffic  7:1-14, 2006.
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Research and Clinical Interests

  • Cell transfection

  • Membrane transport

  • Perfused livers

  • Kinase/Phosphatase assays

  • Subcellular fractionation

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Major Specialized Equipment Items Available

  • Fluorescent spectrophotometers
  • UV-VIS spectrophotometers
  • Scintillation Counter
  • Gel-Electrophoresis (1D & 2D) apparatus
  • Film developer
  • Cell culture facility
  • Fluroescence microscope
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Selected Publications

  1. Webster, CRL, Srinivasulu, U, Ananthanarayanan, M, Suchy, FJ, Anwer, MS. Protein kinase B/Akt mediates cAMP- and cell swelling-stimulated Na+/taurocholate cotransport and Ntcp translocation. J. Biol. Chem. 277:28578-28583, 2002.

  2. Webster, CRL, Blanch, C, Anwer, MS: Role of PP2B in cAMP-induced dephosphorylation and translocation of Ntcp. Am. J. Physiol. 283:G44-50, 2002.

  3. Webster, CRL, Usechak, P, Anwer, MS: Cyclic AMP inhibits bile acid induced apoptosis by blocking caspase activation and cytochrome C release. Am. J. Physiol. 283:G727-736, 2002

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