Education | Laboratory Personnel | General Research Interest | Selected Research Projects
Research and Clinical Interests | Major Specialized Equipment Items Available | Selected Publications
TCSVM Faculty

Mohammed S. Anwer
Distinguished Professor
Associate Dean for Research
Department of Biomedical Sciences
Pharmacology
Phone: 508-839-8788
Fax: 508-839-8787
Email: sawkat.anwer@tufts.edu

Education
DMVH - Munich University - 1980
PhD - Kansas State University - 1973
MS - Dhaka University - 1968

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Laboratory Personnel
Holly Jameson, Senior Research Technician
Christopher Schonhoff, Ph.D., Research Assistant Professor

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General Research Interest
Functional abnormalities associated with various liver diseases lead to accumulation of toxic products (such as bilirubin in jaundice and bile acids) in blood and premature death of liver cells. One of the major determinants is the inability of the liver to properly regulate bile acid transport from blood to bile. Studies are conducted to better understand the mechanisms regulating bile acid transport. Specifically, studies are designed to determine the role of various kinases and phosphatases in the regulation of bile acid transport. Techniques used are cell culture, transfection of cell lines, solute uptake, western blots, protein phosphorylation, etc.

Research Sponsor Interest:

  • Privately Funded Research
  • Federally Funded Research
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Selected Research Projects

  1. McConkey, M., Gillin, H., Webster, C.R.L., Anwer, M.S.:  Cross-talk between PKCζ and PKB in cAMP mediated TC uptake and Ntcp translocation in hepatocytes.  J. Biol. Chem.  279:20882-20888, 2004.
  2. Anwer, M.S.:  Cellular regulation of hepatic bile acid transport in health and cholestasis.  Hepatology  39:581-590, 2004.
  3. Anwer, M.S., Gillin, H., Mukhopadhaya, S., Balasubramaniyan, N., Suchy, F.J., Ananthanarayanan, M.:  Dephosphorylation of Ser-226 facilitates plasma membrane retention of Ntcp.  J. Biol. Chem. 280:33687-33692, 2005.
  4. Sarkar, S., Bananis, E., Nath, S., Anwer, M.S., Wolkoff, A.W., Murray, J.M.:  PKCζ is required for microtubule-based motility of vesicles containing the Ntcp transport.  Volume  7:1-14, 2006.
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Research and Clinical Interests

  • Cell transfection

  • Membrane transport

  • Perfused livers

  • Kinase/Phosphatase assays

  • Subcellular fractionation

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Major Specialized Equipment Items Available

  • Fluorescent spectrophotometers
  • UV-VIS spectrophotometers
  • Scintillation Counter
  • Gel-Electrophoresis (1D & 2D) apparatus
  • Film developer
  • Cell culture facility
  • Fluroescence microscope
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Selected Publications

  1. Schonhoff, C. M, Webster, C.R.L.,  Anwer, M.S.: Cyclic AMP stimulates MRP2 translocation by activating p38a MAPK in hepatic cells. Am J Physiol Gastrointest Liver Physiol. 298:G667-G674, 2010.

  2. Hohenester, S., Gates, A., Wimmer, R., Beuers, U., Anwer, M.S., Rust, C., Webster C.R.L.: Phosphatidylinositol-3-kinase p110γ contributes to bile salt-induced apoptosis in primary rat hepatocytes and human hepatoma Cells. J. Hepatology 53: 918-926, 2010. PMCID: 20675006

  3. Schonhoff, C.M., Ramasamy, U, Anwer, M.S.: Nitric oxide-mediated inhibition of taurocholate uptake involves S-nitrosylation of NTCP. Am. J. Physiol. 300:G364-G370, 2011. PMCID: 21109590

  4. Schonhoff, C.M.,  Webster,C.R.L., Anwer, M.S.: Taurolithocholate induced MRP2 retrieval and MARCKS phosphorylation is mediated via nPKCε in HuH-NTCP cells. Hepatology 52:594A, 2010

  5. Johnston, A.N., Ponzetti, K., Hohenester, S., Anwer, M.S., Webster, C.R.L.: Bile acid induced hepatocyte apoptosis involves a phosphoinositide-3-kinase gamma/protein kinase C delta mediated mitochondrial pathway.. Hepatology 52:452A, 2010